Fig. 2.

SRC regulates CSC-like properties, cell growth and migration/invasion behaviors. A–B Western blot analyses of total proteins from the CAL51 or MDA-MB-231 cells stably expressing vector control (Ctrl) or CA-SRC using the indicated antibodies. The western-blot band intensities of various markers were normalized to the corresponding Actin intensity, and the quantitative values have been provided. C–D Tumorsphere formation ability was analyzed in Ctrl or CA-SRC MDA-MB-231 cells. Representative images of tumorspheres were shown. Scale bars = 100 μm. The quantitation data represent means ± SD with 3 biological replicates. E In vitro cell migration/invasion ability was measured in Ctrl or CA-SRC MDA-MB-231 cells using the Transwell chamber or Transwell chamber containing the Matrigel as barrier. Representative images of migrated/invaded cells were shown. Scale bars = 100 μm. The quantitation data represent means ± SD with 3 biological replicates. F Schematic for tail vein injections of MDA-MB-231 cells, luciferase-labeled cells were captured and analyzed every two-weeks after the injection for 10 weeks. Mice were terminated and the lungs were collected for HE staining at the tenth week. G The quantification data was based on the bioluminescence signal intensities of lung-colonized tumor cells and represent means ± SD. H–I HE staining of sections from lung nodules and the quantification data represent the relative area of lung nodules. n = 6. Scale bar = 100 µm. J Western blot analyses of total proteins from the MDA-MB-231 cells stably expressing shRNA vector control (shCtrl) or shSRC using the indicated antibodies. The western-blot band intensities of various markers were normalized to the corresponding Actin intensity, and the quantitative values have been provided. K–L Tumorsphere formation ability was analyzed in shCtrl or shSRC MDA-MB-231 cells. Representative images of tumorspheres were shown. Scale bars = 100 μm. The quantitation data represent means ± SD with 3 biological replicates. M In vitro cell migration/invasion ability was measured in shCtrl or shSRC MDA-MB-231 cells using the Transwell chamber or Transwell chamber containing the Matrigel as barrier. Representative images of migrated/invaded cells were shown. Scale bars = 100 μm. The quantitation data represent means ± SD with 3 biological replicates. N The quantification data was based on the bioluminescence signal intensities of lung-colonized tumor cells and represent means ± SD. O–P HE staining of sections from lung nodules and the quantification data represent the relative area of lung nodules. n = 6. Scale bar = 100 µm