Skip to main content
. 2023 Jan 12;14:23. doi: 10.1038/s41467-022-35404-0

Fig. 2. A specific G9a inhibitor RK-701 induces the expression of fetal globin.

Fig. 2

a Relative expression of β- and γ-globin genes in HUDEP-2 cells treated with various concentrations of RK-701 or RK-0133114 for 4 days. Amounts of β- and γ-globin RNAs were measured by RT-qPCR. Data are mean ± SD from three independent experiments. b Relative expression of β- and γ-globin mRNAs in CD34+-derived cells treated with RK-701 or hydroxyurea on day 10 of erythroid differentiation. Data are mean ± SD from three independent experiments. c The percentage of γ-globin proteins, relative to total β-like globin proteins (γ-globin + β-globin), in CD34+-derived cells treated with RK-701 or hydroxyurea on day 11 of erythroid differentiation. Data are mean ± SD from three independent experiments. d Flow cytometric analysis of γ-globin expression in CD34+-derived cells treated with RK-701 or hydroxyurea on day 11 of erythroid differentiation. Percentages of HbF-expressing cells were determined by gating HbF-positive cells. Data are mean ± SD from three independent experiments. A representative image of three different analyses is shown. e H3K9me2 levels in CD34+ cells treated with RK-701 or hydroxyurea on day 10 of erythroid differentiation. A representative image of three different analyses is shown. Blots of H3 and H3K9me2 on different gels were derived from the same sample and processed in parallel. f H3K9me2 levels in peripheral blood of mice after treatment with RK-701 for 5 weeks (n = 4–5 mice per group). Blots of H3 and H3K9me2 on different gels were derived from the same sample and processed in parallel. g Fold changes in α-, β-, εy-, and βh1-globin mRNA levels relative to Gapdh in peripheral blood of mice after treatment with RK-701 for 1, 2, 3, 4, and 5 weeks (n = 3–6 mice per group). h Mice were intraperitoneally administered RK-701 over 5 weeks, within each week for 5 consecutive days followed by 2 days off. The body weight of individual mice was measured every 3 or 4 days (n = 4–6 mice per group). P-value was calculated by the one-way ANOVA with Tukey’s post hoc test except for (g) calculated by the one-sided Williams test.