Table 1.
Source and identifier for reagents/resources used
| Reagent or resource | Source | Identifier |
|---|---|---|
| Bacterial and virus strains | ||
| E.coli XL10-GOLD KanR ultracompetent cells | Agilent | 200317 |
| E.coli DH10EMBacY competent cells | Geneva Biotech | DH10EMBacY |
| Chemicals, peptides, and recombinant proteins | ||
| H2N-ESDGG(pY)MDMSKDESID(pY)VPMLDMKGDIKYADIE-OH | New England Peptide | N/A |
| Deuterium oxide 99.9% | Sigma Aldrich | 151882-10X1ML |
| ATP | Sigma | A7699- 1g |
| sodium deoxycholate | Sigma | D6750 |
| Protease inhibitor cocktail | Sigma | 535140 |
| Polyoxyethylene (10) lauryl ether | Sigma | P9769 |
| Phosphatidylserine (Porcine Brain) | Avanti | 840032C |
| Phosphatidylethanolamine (Egg yolk) | Sigma Aldrich | P6386 |
| Cholesterol | Sigma Aldrich | 47127-U |
| Phosphatidylcholine (Egg yolk) | Avanti | 840051C |
| Phosphatidylinositol-4,5-bisphosphate (Porcine Brain) | Avanti | 840046 |
| Sphingomyelin (Egg yolk) | Sigma Aldrich | S0756 |
| Dansyl-phosphatidyl serine | Avanti | 810225C |
| 6-well sticky-side chamber | IBIDI | 80608 |
| Polyethanolamine (PEI) | Polysciences | 24765 |
| Critical commercial assays | ||
| Transcreener ADP2 FI Assay (1000 Assay, 384 Well), | BellBrook Labs | 3013-1K |
| Oligonucleotides | ||
| Forward Primer for D915∗ mutation in p110α- CCTTCATTTTGGGAATTGGAaatCGTCACAATAGTAACATCATGGTGAAAGACG | Sigma Aldrich | oHP006F |
| Reverse Primer for D915∗ mutation in p110α- attTCCAATTCCCAAAATGAAGGTAGCTACACAG | Sigma Aldrich | oHP006R |
| Forward Primer for H1047R∗ mutation in p110α- CAAATGAATGATGCACgTCATGGTGGCTGGACAACAAAAATGG | Sigma Aldrich | MR81f |
| Reverse Primer for H1047R mutation in p110α- TGCATCATTCATTTGTTTCATGAAATACTCCAAAGC | Sigma Aldrich | MR81R |
| Forward Primer for M1043L mutation in p110α- GAGTATTTCATGAAACAAcTGAATGATGCACATCATGGTGGCTGG | Sigma Aldrich | MR82F |
| Reverse Primer for M1043L mutation in p110α- TTGTTTCATGAAATACTCCAAAGCCTCTTGC | Sigma Aldrich | MR82R |
| Forward Primer for G1049R∗ mutation in p110α- ATGATGCACATCATcGTGGCTGGAC | Sigma Aldrich | oHP005F |
| Reverse Primer for G1049R mutation in p110α- ACgATGATGTGCATCATTCATTTGTTTCATGAAATACTC | Sigma Aldrich | oHP005R |
| Forward Primer for N1068KLKR mutation in p110α- ATGCATTGAAACTTAAAAGGTGAgcggccgctcgagtctag | Sigma Aldrich | oHP004F |
| Reverse Primer for N1068KLKR mutation in p110α- CCTTTTAAGTTTCAATGCATGCTGTTTAATTGTGTGGAAGATC | Sigma Aldrich | oHP004R |
| Forward Primer for ΔC mutation in p110α- GGCgccggtaccATGCCTCCACGACCATCATCAGG | Sigma Aldrich | MR80F |
| Reverse Primer for ΔC mutation in p110α- agcggccgcTCAATGATGTGCATCATTCATTTGTTTCATGAAATAC | Sigma Aldrich | MR86R |
| Forward Primer for p110α core mutation in p110α- GGGCgccggtaccAACCGTGAAGAAAAGATCCTCAATCGAG | Sigma Aldrich | oHP008F |
| Reverse Primer for p110α core mutation in p110α- GGATCTTTTCTTCACGGTTggtaccggcGCCCTGAAAATAC | Sigma Aldrich | oHP008R |
| Recombinant DNA | ||
| Human p110α (1-1068) in pACEBac1 vector | Dornan et al.11 | GD102 |
| p110α H1047R in pACEBac1 vector | This paper | MR98 |
| p110α M1043L in pACEBac1 vector | This paper | MR100 |
| p110α G1049R in pACEBac1 vector | This paper | HP15 |
| p110α with N1068KLKR in pACEBac1 vector | This paper | HP14 |
| p110α ΔCter (1–1048) in pACEBac1 vector | This paper | MR106 |
| p110α core (106–1068) in pACEBac1 vector | This paper | HP31 |
| Human p85α (1-724) | Dornan et al.11 | EX20 |
| p110α D915N (1-1068) in pACEBac1 vector | This paper | HP16 |
| p110α D915N and H1047R in pACEBac1 vector | This paper | HP19 |
| p110α D915 and M1043L in pACEBac1 vector | This paper | HP25 |
| p110α D915 and G1049R in pACEBac1 vector | This paper | HP27 |
| p110α D915 and N1068KLKR in pACEBac1 vector | This paper | HP26 |
| Free p85α in pFastBac 1 | This paper | AS01 |
| Software and algorithms | ||
| HDExaminer | Sierra Analytics | http://massspec.com/hdexaminer |
| GraphPad Prism 7 | GraphPad | https://www.graphpad.com |
| Adobe Illustrator | Adobe | https://www.adobe.com/products/illustrator.html |
| COSMIC: Catalogue Of Somatic Mutations In Cancer | Sanger; | https://cancer.sanger.ac.uk/cosmic |
| PyMOL | Schroedinger | http://pymol.org |
| Other | ||
| Sf9 insect cells for expression | Expression Systems | 94-001S |