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. 2023 Jan 12;14(1):19. doi: 10.1038/s41419-023-05553-1

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — A A549 and PC-9 cells were grown for 4 d in 0.5% FSC media in 2D contact coculture with HFL1 cells transfected either with si-scrambled (si-scr) or si-HS2/HS3 (si-HS3) RNAs. A representative photo for coculture of PC-9 cells with distinct round morphology is included. Number of tumor cells (measured as relative cell density) from 3 independent experiments is shown at the graph (absolute number of cells from one representative experiment is in Fig. S9A). B Serum-starved A549 or PC-9 tumor cells were grown in the presence of conditioned media collected from activated HFL1 cells or pre-treated with bleomycin. Relative cell number (%) from three (PC-9) and five (A549) independent experiments is presented. The photo of cells is in Fig. S9B, the absolute number of cells from one representative experiment is in Fig. S9C. C. A549 and PC-9 cells were grown for 4 d in 2D-coculture with HFL1 cells transfected either with si-scr or si-HS2/HS3 in the presence of 1 μg/ml cisplatin in media with 5% FCS. The graph shows the absolute number of cells from one out of three independent experiments. D. Percentage of AnnexinV+ PC-9 or A549 cells treated for 48 h with 1 μg/ml cisplatin in the presence of conditioned media collected from activated HFL1.