Table 1.

MS data collection parameters for a targeted PRM analysis, and the transitions used for quantitation of the tryptic glycopeptides of IgG and HPX.

Glycopeptide	Glycan structure	Mass [m/z]	CS [z]	Polarity	Start (min)	End (min)	NCE	Transitions
G0- EEQYN297STYR		830.001	3	+	1	2.5	12	1141.2–1143.2
G0N-EEQYN297STYR		897.694	3	+	1	2.5	12	1242.6–1244.6
G0FN-EEQYN297STYR		946.38	3	+	1	2.5	12	1317.3–1319.3
G0F-EEQYN297STYR		878.687	3	+	1	2.5	12	1215.7–1217.7
G1- EEQYN297STYR		884.018	3	+	1	2.5	12	1141.2–1143.2
G1N- EEQYN297STYR		951.712	3	+	1	2.5	12	1242.6–1244.6
G1FN- EEQYN297STYR		1000.398	3	+	1	2.5	12	1317.3–1319.3
G1F- EEQYN297STYR		932.704	3	+	1	2.5	12	1215.7–1217.7
HexNAc(1)Hex(1)Neu5Ac(1) T24PLPPTSAHGNVAEGETKPDPVTER		843.6	4	+	2.4	4	20	905.5–907.0
HexNAc(1)Hex(1)Neu5Ac(2) T24PLPPTSAHGNVAEGETKPDPVTER		916.4	4	+	2.4	4	20	905.5–907.0
IgG peptide GPSVFPLAPSSK		593.8	2	+	2.6	4	35	417.9–419.9, 699.0–701.0, 846.0–848.0

*HexNAc: blue rectangle (GlcNAc), yellow rectangle (GalNAc).

Hex: green circle (Man), yellow circle (Gal).

Red triangle (Fuc).

Purple diamond (Neu5Ac).

The glycan structure of the peptides is shown.*