Table 1.

Experimental parameters and mean values of Cbl forms in different organs.

Experiment #	All animals (n)	Linagliptin (n)	Treatment duration (Days)	Organ	Cobalamin (µg/kg chow)	Cobalamin Type	Mean Control ± SD [a.U.]	Mean Linagliptin ± SD [a.U.]	p TT	p KW
1	38	14	84	Kidney	30	OH	0.96 ± 0.51	1.74 ± 0.80	0.0039	0.0020
						CN	0.12 ± 0.06	2.37 ± 0.78	< 0.0001	< 0.0001
	10	5	84	Liver	30	OH	0.62 ± 0.19	0.60 ± 0.41	0.9230	0.2620
						CN	0.06 ± 0.01	0.15 ± 0.04	0.0011	0.0040
	38	14	84	Heart	30	OH	0.24 ± 0.15	0.22 ± 0.11	0.9230	0.9760
						CN	0.02 ± 0.00	0.04 ± 0.02	0.0019	0.0003
2	10	5	28	Kidney	Lina: 150 Controls: 50	OH	0.66 ± 0.36	1.10 ± 0.31	0.0459	0.1090
						CN	0.61 ± 0.26	1.17 ± 0.21	0.0021	0.0040
3	10	5	4	Kidney	150	OH	1.70 ± 0.66	1.46 ± 0.52	0.4980	0.2620
						CN	1.59 ± 0.16	1.76 ± 0.44	0.4200	0.5220
	10	5	4	Heart	150	OH	0.10 ± 0.03	0.12 ± 0.06	0.4930	0.7490
						CN	0.05 ± 0.03	0.03 ± 0.02	0.2690	0.1090
	10	5	4	Liver	150	OH	0.26 ± 0.09	0.28 ± 0.14	0.8030	0.8730
						CN	0.12 ± 0.04	0.09 ± 0.04	0.1230	0.0782
4	8	4	14	Kidney	30	OH	1.81 ± 0.30	1.57 ± 0.26	0.0204	0.0546
						CN	1.80 ± 0.80	1.43 ± 0.17	0.0864	0.5460
	8	4	14	Spleen	30	OH	0.10 ± 0.02	0.11 ± 0.02	0.1280	0.1870
						CN	0.05 ± 0.01	0.06 ± 0.01	< 0.0001	0.0001
	8	4	14	Heart	30	OH	0.06 ± 0.02	0.08 ± 0.01	0.0262	0.0288
						CN	0.02 ± 0.01	0.03 ± 0.01	0.5000	0.6780

a.U. arbitary unit; CN cyano; KW Kruskal–Wallis; Lina linagliptin; OH hydroxy; SD standard deviation; TT Welch t-test.

An overview of experiments (Exp#1–4) conducted in mice to determine the relative Cbl amount in different organs at different Vit-B12 diets and treatment durations under linagliptin and placebo. Significant p values (< 0.05) are in bold.