Table 2.
Number of plants analyzed and RPGR% in back-cross generations during the marker assisted introgression of Saltol QTL
| Generation | Tested and selected plants | Onice × IR64-Saltol | Vialone nano × IR64-Saltol |
|---|---|---|---|
| BC1F1 | Tested plants | 330 | 220 |
| Heterozygous plants at flanking markers | 78 | 39 | |
| Selected plants in background selection | 12 | 10 | |
| RPGR (Os01 excluded) | 46–72% | 45–61% | |
| BC2F1 | Tested plants | 166 | 208 |
| Heterozygous plants at flanking markers | 57 | 74 | |
| Selected plants in background selection | 17 | 18 | |
| RPGR (Os01 excluded) | 75–90% | 73–89% | |
| BC3F1 | Tested plants | 242 | 276 |
| Heterozygous plants at flanking markers | 63 | 144 | |
| Selected plants in background selection and selfed | 14 | 15 | |
| RPGR (Os01 excluded) | 89–98% | 91–98% | |
| BC3F2 | Tested plants | 500 | 468 |
| Homozygous plants at flanking markers | 119 | 99 | |
| Selected plants in background selection and selfed | 21 | 19 | |
| RPGR (Os01 excluded) | 92–100% | 93–100% | |
| BC3F3 | Tested plants | 133 | 144 |
| Homozygous plants at flanking markers | 62 | 48 | |
| Selected plants in background selection and selfed | 14 | 12 | |
| RPGR (Os01 excluded) | 93–100% | 94–100% | |
| BC3F4 | Tested plants | 14 | 12 |
| Homozygous plants at flanking markers | 14 | 12 | |
| Selected and selfed plants | 14 | 12 |
Tested plants indicate the number of analyzed plants, heterozygous/homozygous plants at flanking markers indicate the number of plants selected in foreground selection that have the marker alleles flanking Saltol locus in heterozygous or homozygous condition. RPGR% = 1 − (KASP heterozygous (not included in Saltol locus) /total KASP markers)* 100