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Recognition of monomeric and oligomeric C2IIa by anti-C2II707–721 antiserum. Both C2II and C2IIa oligomers (200 ng of each protein) were subjected to SDS–3 to 12.5% PAGE without (lanes 1 and 2) and with (lanes 3 and 4) prior heating at 95°C. Proteins were blotted onto a nitrocellulose membrane and detected with anti-C2II707–721 antiserum. As a control, the same blot was probed with anti-C2II-D4 antiserum.
