TABLE 1.
Analysis of various C2II antiseraa
| Protein | Test result with indicated antiserum
|
|||||
|---|---|---|---|---|---|---|
| Anti- C2II | Anti- C2II-D1 | Anti- C2II182–196 | Anti- C2II-D3 | Anti- C2II-D4 | Anti- C2II707–721 | |
| C2II | + | + | + | + | + | + |
| C2IIa | + | + | + | + | + | + |
| C2II-D1 | − | + | + | − | − | − |
| C2II-D3 | + | − | − | + | − | − |
| C2II-D4 | + | − | − | − | + | + |
a
C2II, C2IIa, C2II-D1, C2II-D3, and C2II-D4 (200 ng each) were subjected to SDS–12.5% PAGE and blotted to a nitrocellulose membrane. Proteins were detected with anti-C2II, anti-C2II182–196, anti-C2II-D1, anti-C2II-D3, anti-C2II-D4, and anti-C2II707–721 antisera. +, protein was recognized by antiserum; −, protein was not recognized by antiserum.