Figure 5.

Effects of chronic stress on enteric glia population and neurons in proximal colon. Western blot analysis of specific glia markers, glial fibrillary acidic protein (GFAP) and S100 calcium-binding protein B (S-100B), and neurons (protein gene product 9.5 [PGP 9.5]) in proximal colon of stressed and control mice (n = 7-8/group). Protein fold-change was calculated for each sample with reference to the average of the target protein to β-actin ratio of the control group. The protein expression of enteric glia markers were significantly decreased in proximal colon after chronic stress when compared with control mice. Data represent mean ± SEM, *P < 0.05 by Mann Whitney test.