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. 2022 Oct 14;98(1):17–25. doi: 10.1016/j.abd.2021.12.007

Figure 1.

Figure 1

MiR-181a depletion mitigates H2O2-induced cellular senescence and oxidative stress. Assays were conducted in HFF with different treatments (with or without H2O2; transfection of miR-NC or miR-181a inhibitor). (A) RT-qPCR analysis of miR-181a level. (B) CCK-8 assay for evaluating cell viability. (C) SA-β-gal staining for assessing SA-β-gal activity. Cell pictures were imaged at 50× magnification. (D‒G) Western blotting for measuring protein levels of senescence markers. (H‒J) Measurement of the activities of SOD, GPx and CAT in HFF. *p < 0.05, **p < 0.01, ***p < 0.001.