Skip to main content
. Author manuscript; available in PMC: 2023 Jan 13.
Published in final edited form as: Ocul Surf. 2019 Oct 25;18(1):1–12. doi: 10.1016/j.jtos.2019.10.007

FIGURE 2: Rarefaction curves of publicly available metagenomic samples from healthy subjects, retrieved from the NCBI Sequence Read Archive (SRA), and analyzed using the MG-RAST (metagenomics Rapid Annotation using Subsystem Technology)123 bioinformatics pipeline:

FIGURE 2:

(A) Conjunctival microbiome (SRR3033169; post-quality control sequence count: 4,680,480) and (B) oral salivary microbiome (ERR1474610; post-quality control sequence count: 3,902,367). The respective curves demonstrate the vast discrepancy in species count per read number between the two samples, reflecting the paucimicrobial nature of the conjunctiva compared to the gastrointestinal tract. The MG-RAST-derived alpha-diversity scores of 7 and 84, respectively, are measures of species richness within the samples.