TABLE 2.
Amino acid sequences of Ca-MP65, Sc-MP65, and the peptides used to map Ca-MP65 epitopes
| Protein or peptide (amino acids) | Sequencea |
|---|---|
| Proteins | |
| Ca-MP65 (142–160) | SSSQIASEIAQLSGFNVIR |
| Sc-MP65 (152–170) | STAQVASDLEQLTGFDNIR |
| Ca-MP65 (182–204) | IFAGIFDVSSITSGIESLAEAVK |
| Sc-MP65 (192–214) | LFLGIYYVDKIQDAVDTIKSAVE |
| Peptides | |
| T1a | IFAGIFDVSSITSGI |
| T1b | SSITSGIESLAEAVK |
| T2a | SSSQIASEIAQLSGF |
| T2b | IASEIAQLSGFNVIR |
| Aligned peptides | |
| T1a | IFAGIFDVSSITSGI |
| T1b | SSITSGIESLAEAVK |
| T2a | SSSQIASEIAQLSGF |
| T2b | IASEIAQLSGFNVIR |
| Amino acid motifb | |
| Position | 12345678910 |
| Amino acid | XIXSXIXXLX |
a
Bold type indicates amino acids relevant for TCC recognition. Italic type indicates the Saccharomyces cerevisiae MP65 corresponding sequence.
b
Positions 1 to 10 indicate the amino acid position in the motif sequence. X indicates an amino acid not relevant for TCC recognition.