Figure 1.

Experimental workflow.

Uninjured WT rats, CMT1A rats, and WT rats exposed to sciatic nerve crush were used for distal sciatic nerve collection (1 nerve/rat). Protein trypsin digests were separated and analyzed by mass spectrometry using SWATH LC-MS/MS. Identified proteins were compared between (i) CMT1A vs. WT group (compa1) and (ii) SNC vs. WT group (compa2). Using Gene Ontology terms, proteins were mapped into biological processes for further analysis. CMT1A: Charcot-Marie-Tooth-1A; SNC: sciatic nerve crush; SWATH LC-MS/MS: sequential window acquisition of all theoretical fragment ion spectra liquid chromatography and mass spectrometry; WT: wild-type.