Figure 6.

Pathological analysis of cerebral cortex supplied by the left middle cerebral artery (affected side).

Changes in brain tissue on the affected side were visualized after reperfusion using hematoxylin and eosin staining in the control (A), 0- (B), 6- (C), 12- (D), 24- (E), 72- (F), and 168-hour (G) groups. Scale bars: 50 μm. The vessel walls and brain tissue were severely damaged at 12 to 24 hours after reperfusion and the number of cells in the cortex was decreased. Some cortical neurons were necrotic and degenerated with pyknotic and hyperchromatic nuclei. Some nerve fibers were loosely arranged and irregular with sieve-like structures and many vacuoles. However, 72 hours after reperfusion, the cells had recovered. (H) Schematic diagram of the left middle cerebral artery-supplied cortex.