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. 2022 Oct 11;18(6):1371–1377. doi: 10.4103/1673-5374.355978

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OxPAPC treatment does not influence the effect of argon preconditioning on HO-1 and HSP-70.

(A, B) Analysis of HSP70 (A) and HO-1 mRNA (B) expression using qPCR. Fold induction of HSP70 and HO-1 mRNA expression compared to GAPDH in relation to untreated cells after treatment with rotenone, preconditioning with argon, and inhibition with OxPAPC. Argon attenuates the rotenone-induced release of the cytokine IL-8 and OxPAPC counteracts this effect. (C) Analysis of IL-8 mRNA expression using qPCR. Fold induction of IL-8 mRNA expression compared to GAPDH in relation to untreated cells after treatment with rotenone, preconditioning with argon, and inhibition with OxPAPC. Between-group comparisons were performed with a one-way analysis of variance using the post hoc Holm Sidak test (n = 6; mean ± SD; *P < 0.05, ***P < 0.001). GAPDH: Glyceraldehyde 3-phosphate dehydrogenase; HSP70: Heat shock protein 70; IL-8: interleukin-8; qPCR: real-time polymerase chain reaction.