IgG+ B cells from MPXV recoverees bind A35R and H3L antigens
(A) Flow cytometry gating strategy for staining of A35R- and H3L-specific IgG+ B cells. Representative plots for MPXV recoveree (Mpx01), VPXV (Vpx05) and uninfected <45yo (<45yo-2) are shown.
(B) Frequency of A35R-specific IgG+/CD19+ cells of MPXV recoverees are in red (n = 11), VPXV are in purple (n = 6) and uninfected <45yo are in gray (n = 3). Statistical analysis was performed between MPXV and VPXV groups using Unpaired t test. ∗p< 0.05, ∗∗p< 0.01.
(C) The same as (B), but for H3L-specific IgG+/CD19+ cells.