FIG. 5.

Analysis of transcription from promoter-lacZ fusions during growth in CLR medium. 8325-4 (wild type) containing the fusion indicated was grown in CLR medium (▴), CLR with 20 μM manganese (⋄), and CLR with 20 μM iron sulfate (○). Transcription of the lacZ fusions in MJH001 (perR) (▪) is shown on each graph. Samples were removed at the times indicated and sampled for β-galactosidase activity. The strains grew with slightly different growth rates as indicated in Fig. 2, but for reasons of clarity only the changes in β-galactosidase activity are shown. The lacZ fusions shown are MJH002 (ahpC-lacZ), MJH003 (bcp-lacZ), MJH004 (ftn-lacZ), MJH005 (fur-lacZ), MJH006 (katA-lacZ), MJH007 (mrgA-lacZ), MJH008 (perR-lacZ), and MJH009 (trxB-lacZ), and these same fusions in the perR mutant background are MJH102, MJH103, MJH104, MJH105, MJH106, MJH107, MJH108, and MJH109, respectively.