Table 3.

Timeline for the synthesis of the Omicron Spike construct

	Day	Activity
Bench work	1	Strategy finalized
	2	Primers ordered
	3
	4
	5	Primers received
	6	Fragment PCRs round 1
	7	OE and Gibson assembly round 1
	8	Col PCR 1
	9	Plasmid prep, sent for sequencing
	10
	11
	12
	13	Sequencing results; Fragment PCRs round 2
	14	Round 2 cloning
	15
	16	Col PCR 2
	17	Plasmid prep, sent for sequencing
	18
	19
	20	Sequencing results
	21	Fragment PCRs round 3
	22	Round 3 cloning
	23	Col PCR 3
	24
	25
	26	Plasmid prep, sent for sequencing
	27
	28
	29	Sequencing results for final clone