OE1
PCR fragments after column clean-up (100 ng/kb each)	10 µl
2 × Q5 mix	12.5 µl
Water	2.5
Total	25 µl
PCR Programme for OE1:
94°C: 30 s; [94°C: 15 s; 72°C: (−0.5°C/cycle) 30 s/kb] for 9 cycles; [94°C: 15 s; 67°C: (−0.5°C/cycle) 15 s, 72°C: 30 s/kb] for 5 cycles; 72°C: 2 min; Hold at 10°C
OE2
OE1 product	2 µl
2 × Q5 mix	12.5 µl
2 µM FP	0.35 µl
2 µM RP	0.35 µl
Water	9.8 µl
Total	25 µl
PCR Programme for OE2:
94°C: 30 s; [94°C: 15 s ; 67°C: (−0.5°C/cycle) 15 s; 72°C 30 s/kb] for 17 cycles; [94°C: 15 s; 59°C: 15 s; 72°C: 30 s/kb] for 23 cycles; 72°C: 2 min; Hold at 10°C