Fig. 5.

Expression of human prion protein, its peptides fused with GST tag and Hsp60 via SDS-PAGE (left) and Western blot (right) A, Hsp60 protein; B, huPrP-GST protein; C, PrP125-GST protein; D, PrP174-GST protein; E, PrP180-GST protein; lane 1: low range weight protein marker, 97–14.4 kDa (Cytiva) (SDS-PAGE) and Pierce™ prestained protein molecular weight marker, 20–120 kDa (Western blot); lane 2: induced E. coli BL21(DE3); lane 3–6 of A total cellular protein, soluble, insoluble fractions of E. coli BL21(DE3)/pET22b-hsp60, respectively; lane 3–5 of B, C, D, E total cellular protein, soluble, insoluble fractions of induced E. coli BL21(DE3)/ pGEX-5X1, respectively; lane 6–8: total cellular protein, soluble, insoluble fractions of target proteins, respectively