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. 2023 Jan 13;13:715. doi: 10.1038/s41598-023-27965-x

Figure 2.

Figure 2

Protection of aged mice following prime-only vaccination (5 μg HA). (a) Pre-infection day 28 H1-specific serum ELISA total IgG, (b) isotype subclass specific IgG2a and (c) IgG1 of aged BALB/c mice vaccinated with 5 µg Cal/09 rHA protein in combination with 50 BECC438 or 50 µg BECC470 adjuvant in prime only schedule (mean ± SEM). (b) Area under the curve (AUC) (mean ± standard error) of ELISA curves for (d) total IgG (*p = 0.0258), (e) IgG2a (*p = 0.0484) and (f) IgG1 (*p = 0.0259). (g) 7-day weight loss in BALB/c mice (5 per group) after infection with 32,000 PFU of NL/09 (mean ± SEM). (h) Virus titer of lung homogenate 7-days post infection (mean ± SEM) with (i) pathology inflammation scoring of lung histology slides (mean ± SEM). Prism 9 used to calculate mean ± SEM and AUC ± standard error of the mean.