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. 2023 Jan 13;6:42. doi: 10.1038/s42003-023-04431-y

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Above: comparisons of 2D class averages between polyC9 alone and aE11-Fab/polyC9. Below: 3D density map comparison between top-down and side orientations of the full cryo-EM maps of the aE11-Fab/polyC9 complex (transparent grey surface) and unbound polyC9 (blue). Left: focused refinement of C9 trimer (blue) with aE11-Fab (red) positioned in the global map of aE11-Fab/polyC9 (transparent grey). Right; unbound polyC9 alone (EMDB: 7773). b Focused view of the boxed region from (a; oblique and side views), three C9 subunits, and two aE11-Fabs are shown as isosurfaces. c Corresponding cartoon representation of the atomistic model derived from (b). The direction of MAC assembly is indicated by the arrow. d The positions of the heavy (H) and light (L) chains of aE11-Fab, in red and beige, respectively, and domains of the C9 subunit: MACPF (grey) and ancillary domains TSP (blue), LDLRA (green), and EGF (purple). e Top-down view of the aE11-Fab wedged between the two C9 protomers, defined as leading (light blue) and lagging (grey) subunits. Black arrow shows direction of MAC assembly.