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. 2023 Jan 13;6:42. doi: 10.1038/s42003-023-04431-y

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Concentration series and effective half-maximal concentration of MAC haemolytic assay with C9 variants (n = 3; mean and standard error of the mean [SEM] are plotted). b Negative-staining electron microscopy of polyC9 variants. c Nano-differential scanning fluorimetry measurements of apparent thermal stability. d Maximal SPR response of aE11 IgG binding versus concentration of polyC9 and variants (n = 2). e Maximal binding of aE11 IgG (arbitrary response units) at 20 nM of polyC9 and variants (n = 2). Individual data points and their means are plotted.