Table 1.
Cryo-EM data collection, refinement, and validation statistics.
| aE11-Fab/polyC9 (EMDB-27385) (PDB 8DE6) | aE11-Fab/polyC9 (EMDB-28863) | |
|---|---|---|
| Data collection and processing | ||
| Magnification | 105,000 | 105,000 |
| Voltage (kV) | 300 | 300 |
| Electron exposure (e–/Å2) | 52.8 | 52.8 |
| Defocus range (μm) | –0.5 to –2.0 | –0.5 to –2.0 |
| Pixel size (Å) | 1.4 | 1.4 |
| Symmetry imposed [after expansion] | [C1] | C22 |
| Initial particle images (no.) [symmetry expand] | 48248 [1,061,456] | 48248 |
| Final particle images (no.) [symmetry expand] | 10,061 [221,346] | 7783 |
|
Map resolution (Å) FSC threshold |
3.2 0.143 |
4.2 0.143 |
| Map resolution range (Å) | 3.0 to 5.0 | 3.8 to 7.0 |
| Refinement | ||
| Initial model used (PDB code) | 6DLW/3BAE | |
|
Model resolution (Å) FSC threshold |
3.35 0.5 |
|
| Map sharpening B factor (Å2) | –134.7 | |
| Model composition | ||
|
Non-hydrogen atoms Protein residues Ligands |
13243 1659 12 |
|
| B factors (Å2) | ||
|
Protein Ligand |
22.40/163.79/79.49 69.84/137.08/100.74 |
|
| R.m.s. deviations | ||
|
Bond lengths (Å) Bond angles (°) |
0.010 0.895 |
|
| Validation | ||
|
MolProbity score Clashscore Poor rotamers (%) |
1.81 4.54 2.53 |
|
| Ramachandran plot | ||
|
Favoured (%) Allowed (%) Disallowed (%) |
96.02 3.98 0.00 |
|