TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Derivation and propertiesa | Reference(s) |
|---|---|---|
| Strains | ||
| A66 | Type 3 parent strain; Cps+ | 4 |
| AM161 | pJD330 × A66, insertion-duplication mutation downstream of cps3S; Emr Cps+ | This study |
| AM179 | pCV182 × A66, Δcps3UMtnpA1; Cps+ | This study |
| AM188 | A66 cps3D5(V283E); Cpsr (20%) | This study |
| AM199 | pJD369 × A66, insertion-duplication mutation downstream of cps3D (polar on cps3S); Emr Cps− | This study |
| AM201 | AM188 with repaired cps3D5 mutation; Cps+ | This study |
| AM1000 | D39 Δcps2ABCDETFGHI; Cps− | This study |
| D39 | Type 2 parent strain; Cps+ | 4 |
| JD600 | WU2; Strr Cps+ | 20 |
| JD614 | JD600 cps3D3 (mutation located between bp 885 and 1802); Strr Cpsr (<6%) | 19, 20 |
| JD692 | JD600 cps3D4 (mutation located between bp 1027 and 1802); Strr Cpsr (<0.6%) | 19, 20 |
| JD770 | pJD330 × WU2, insertion-duplication mutation downstream of cps3S; Emr Cps+ | 19, 20 |
| JD908 | pJD369 × WU2, insertion-duplication mutation downstream of cps3D (polar on cps3S); Emr Cps− | 19, 20 |
| JY1060 | WU2 pgm-1(K381T); Cpsr (20%) PGMr | 26 |
| R36A | D39 serially passaged 36 times in rabbit anti-type 2 serum, Δcps2ABCDETFGHI; Cps− | 4, 31 |
| WU2 | Type 3 parent strain; Cps+ | 10 |
| Plasmids | ||
| pJY4163 and pJY4164 | Cloning vectors for insertion-duplication mutagenesis; lack origin of replication for S. pneumoniae; multiple cloning site in opposite orientations in pJY4163 and pJY4164; Emr | 58 |
| pCV182 | pJY4164::3.14-kb [Sau3AI-PstI (‘cps3DSU’), SacI-HindIII (‘tnpA-plpA’)]; Emr | 27; this study |
| pJD330 | pJY4163::2.38-kb Sau3AI (‘cps3DSU’); Emr | 20 |
| pJD369 | pJY4164::0.575-kb PvuII-RsaI (‘cps3DS’); Emr | 20 |
| pJD380 | pJY4164::0.356-kb Sau3AI-SspI fragment from pJD330 (‘cps3D’); Emr | 20 |
a
Emr, erythromycin resistant; Strr, streptomycin resistant; Cps+, encapsulated; Cps−, nonencapsulated; Cpsr (percentage of parental level), reduced capsule production; PGMr, decreased PGM activity.