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High-inoculum bactericidal assay. The decrease (log10) in the number of bacteria resulting from bacteriolysis in the presence of complement and anti-NspA MAb AL12, compared to the number of control bacteria incubated with antibody alone or antibody with heat-inactivated complement, is shown. The numbers in parentheses indicate the BC50 concentrations of AL12 for each strain measured in the low-inoculum bactericidal assay.
