Figure 4.

Individual ICH stains were used to map different cell types. A, Example section (Level I, further shown in Figs. 22, 23) Nissl section with dots used to represent each cell type (orange represents ChAT; lime green represents TH; dark green represents melanin; pink represents TpOH). Boxes represent location of B–D. B–D, Adjacent section stained blackish purple with DAB for ChAT, TH, or TpOH, respectively. Brown cells are endogenous pigmented melanin. D, Most of the TH⁺ NTS neurons do not contain melanin (examples marked with green arrows), but one cell contains both melanin and TH (solid black arrow), and some cells with melanin are TH^– (hollow arrows). Scale bars, 100 μm.