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. Author manuscript; available in PMC: 2023 Jun 17.
Published in final edited form as: Science. 2022 Jun 16;376(6599):1343–1347. doi: 10.1126/science.abn3027

Fig. 2. METRNL binds to the extracellular domain of KIT.

Fig. 2.

(A) Conditioned supernatants (SN) were prepared from HEK-293 cells transfected with expression plasmids encoding human KIT-extracellular domain-Fc fragment fusion protein (KIT-ECD-Fc), METRNL, stem cell factor (SCF), and/or vascular endothelial growth factor A (VEGFA). Pull-down of KIT-ECD-Fc using protein G-coated beads copurified METRNL and SCF. Exemplary results from 3 experiments. (B) Protein-protein interaction analysis by microscale thermophoresis. Binding curves illustrate the interaction of human METRNL and SCF with human KIT-ECD-Fc. Human VEGFA did not interact with KIT-ECD-Fc, nor did METRNL with an IgG1-Fc control protein. Data are means ± SEM from 3–4 technical replicates; 2 additional experiments yielded similar results.