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. Author manuscript; available in PMC: 2023 Jun 17.
Published in final edited form as: Science. 2022 Jun 16;376(6599):1343–1347. doi: 10.1126/science.abn3027

Fig. 3. METRNL and SCF signaling through KIT in endothelial cells.

Fig. 3.

(A) Expression of P-KIT (Y568/Y570) and α-tubulin in human coronary artery endothelial cells (HCAECs) stimulated for 5 min with METRNL (10 ng/mL) or stem cell factor (SCF, 10 ng/mL) in the absence or presence of recombinant Fc fragment (10 μg/mL) or KIT-extracellular domain-Fc fragment fusion protein (KIT-ECD-Fc, 10 μg/mL). Exemplary results from 3 experiments. (B) BrdU incorporation and recovery after scratch injury of HCAECs stimulated with METRNL, SCF, or vascular endothelial growth factor (VEGFA, 25 ng/mL) in the absence or presence of KIT-ECD-Fc. Data are means ± SEM from 4 experiments. (C) Principal component analysis of phosphoproteome data sets from HCAECs stimulated with METRNL, SCF, or VEGFA (4 technical replicates per condition).