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. Author manuscript; available in PMC: 2023 Jan 13.
Published in final edited form as: Cell Rep. 2022 Dec 20;41(12):111860. doi: 10.1016/j.celrep.2022.111860

Figure 5. Average action potentials per light pulse metric shows specificity in circuit strength based on claustrum neuron output target region and claustrum projection neuron subtype.

Figure 5.

(A) Average action potentials (APs) per light pulse stimulation of each voltage trace (for all light intensities) revealed differences in circuit strength across frontal cortical trans-claustral circuits depending on type I and II claustrum projection neuron target (Kruskal Wallis test for multiple comparisons: p < 0.0001; n = 570 type I cells and n = 525 type II cells total).

(B and C) Clustered frontal cortico-claustro-cortical circuits were detected by subgraph extraction (cluster marked in black) for type I and type II claustrum neuron subtypes. Type I: detected subgraph from ACC and plPFC to PtA and V1/V2. Type II: detected subgraph from ACC and plPFC to ACC, plPFC, PtA, and V1/V2. Detected subgraphs p < 0.001 under permutation test.

(D) Select frontal cortico-claustro-cortical circuits preferentially activated type II neurons compared with type I neurons: ACC > CL > ACC (Wilcoxon rank-sum test: p = 0.027); ACC > CL > plPFC (p < 0.0001); ACC > CL > ilPFC (p = 0.003); ACC > CL > OFC (p = 0.006); ACC > CL > V1/V2 (p = 0.016); ACC > CL > PtA (p = 0.009); plPFC > CL > V1/V2 (p = 0.041); ilPFC > CL > ilPFC (p = 0.016); ilPFC > CL > V1/V2 (p = 0.215); and OFC > CL > RSC (p = 0.015). n = 15 cells in each circuit for each subtype. n = 1,050 cells total. Error bars: standard error of the mean.