Figure 4.

Neural stem and progenitor cells generated at several stages of cortical organoid development. Brightfield and fluorescent images of (A) 2D NSC populations harvested from early neural spheroids and (B) 2D neural progenitor cell (NPC) populations from differentiating cortical organoids. (C) Schematic workflow highlighting the utility of NSCs and NPCs that can be propagated and maintained in culture to generate frozen stocks or assayed for cell characterization & compound testing. Scale bar = 200μm.