Figure 3.
MAIT cells are recruited into the inflamed skin
(A) Ki67 expression by MAIT cells. Data are from two (n = 5) independent experiments. Wilcoxon test.
(B) Parabiosis protocol (left) and CD45.2/2 and CD45.1/2 staining (middle). Percentage of partner-derived MAIT, ɣδ T, and mainstream T cells in the skin (right) at steady-state and in the wound and control sites 4 days after excision. Data are from three independent experiments (nsteady state+control = 9; nexcision = 5). Sídák multiple comparison test. Please also see Figure S3A.
(C) Tissue residency and circulating36 signature scores on non-cycling MAIT17 cells. Tukey’s multiple comparison test.
(D) CD69 and CD103 expression by MAIT cells. Pooled data from six independent experiments (nCD69 = 23; nCD103 = 27). Wilcoxon test.
(E) Graft protocol and CD45.2 and CD45.1 staining (left). CD45.2 donor cell frequency in MAIT and ɣδ T cells from the donor skin (D0) and after 6 days in the graft (middle). Absolute number of recipient CD45.1+ MAIT cells in grafts from D0, D6, and D12 (right, grafts from same donor are linked). Pooled data from two independent experiments (nD0 = 5; nD6 = 3/6; nD12 = 6). Mann-Whitney and Wilcoxon tests as appropriate.
(F) Example of Kaede green and red expression (left) and frequency of photoconverted cell (right) in skin MAIT and ɣδ T cells. Pooled data from two independent experiments (nD0 = 4, nD2 = 5). Paired t test.
(G) The number of MAIT cells in the inguinal and brachial LNs draining the wound or the control sites. Pooled data from two independent experiments (n = 6). Paired t test. Please also see Figures S3B and S3C.
(H) Numbers of MAIT cells (ratio wound over control sites) 4 days after excision in FTY720- or PBS-treated mice. Pooled data from two independent experiments (nPBS = 5; nFTY720 = 6). Mann-Whitney test. Please also see Figure S3D.