Figure 4.

MAIT cell recruitment and skin healing are independent of MR1

(A) Grafts were performed on Mr1^+/+ animals. MAIT cell staining (left) and numbers (right) in Mr1^−/− and Mr1^+/+ grafts before transplant (D0) and longitudinally after grafting. Pooled data from 2 independent experiments (Mr1^+/+: n_D0 = 3, n_D6,12 = 4; Mr1^−/−: n_D0 = 3, n_D6 = 4, n_D12 = 6). Unpaired t test.

(B) Mr1^−/− and Mr1^+/+ mice were parabiosed for 5 weeks. MAIT cell staining in the skin of the Mr1^−/− parabiont skin (left) and numbers at wound and control sites (right) for Mr1^−/− and Mr1^+/+ parabionts and Mr1^−/− control mice. Pooled data from two independent experiments (n_Mr1⁻_control = 3; n_Mr1⁻_parabiont = 9; n_Mr1⁺_parabiont = 9). Tukey multiple comparison test.

(C) Percent of wound closure in Mr1^−/− and Mr1^+/+ parabionts and control Mr1^−/− mice. Pooled data from two independent experiments (n_Mr1^−/−_alone = 3; n_Mr1^−/−_paired = 8; n_Mr1^+/+_paired = 8). Mann-Whitney and Wilcoxon tests as appropriate.

(D) Mean fluorescence intensity of GFP expression on MAIT cells at wound and control skin sites in Nr4a1-GFP animals. Pooled data from two independent experiments (n = 6). Paired t test.

(E) MAIT cell numbers at wound and control sites 4 days after transfer into Cd3e^−/−Mr1^+/+ and Mr1^−/− mice. Pooled data from two independent experiments (n_Mr1^+/+ = 4; n_Mr1^−/− = 4). Mann-Whitney test. Please also see Figures S4A–S4C.

(F) Longitudinal follow-up of wound surface of transferred Cd3e^−/−Mr1^+/+ and Mr1^−/− mice and non-transferred Cd3e^−/−Mr1^+/+ control mice. Pooled data from two independent experiments with one blinded (n_WithTransfer = 4/4; n_{WithoutTransfer} =8). Please also see Figure S4D.