Figure 5.

CXCR6 is necessary for MAIT cell recruitment into the skin

(A) MAIT and ɣδ T cell staining (left) and numbers (wound over control sites) (right) in skin control and wound (D4) sites following Ptx injection. Pooled data from two independent experiments (n = 6). Mann-Whitney test. Please also see Figures S5A and S5B.

(B) Chemokine receptor gene expression by non-cycling MAIT17 cells from integrated single-cell datasets as in Figure 1B.

(C) CXCR6 expression by MAIT cells. Data are representative of 10 independent experiments.

(D) CXCL16 protein quantity in total skin lysate from wound (D4) or steady-state skin of Mr1^+/+ and Mr1^−/− mice. Pooled data from two independent experiments (n = 4). Mann-Whitney test.

(E) MAIT cell staining (left) and numbers (right) in control and wound (D4) skin sites following ɑ-CXCL16 or isotype control i.p. injection. Pooled data from two independent experiments (n = 7/10). Mann-Whitney test.

(F) Expanded MAIT cells were deleted for Cxcr6 by CRISPR-Cas9 modification. Congenic marker and CXCR6 expression on the injected pool (top left) or recovered cells (bottom left). Quantitation of recovered Cxcr6^−/− cells (ratio of Cxcr6^−/− over Cxcr6^+/+) at different sites (right). Pooled data from two independent experiments (n_injection = 2; n_other = 6). Tukey’s multiple comparison test.