Skip to main content
. 2023 Jan 10;56(1):78–92.e6. doi: 10.1016/j.immuni.2022.12.004

Figure 6.

Figure 6

MAIT cell-derived Areg exerts a tissue repair function

(A) Representative immunofluorescence images of wounds from Mr1+/+ and Mr1−/− animals (DAPI in blue, K14 in green) (left). Scale bar represents 100 μm. The epidermal tongues are underlined with white dashed lines and their length is quantified (right, D2/D4, 2 tongues per slide). Pooled data from one (D2: n = 3) and two independent experiments (D4: n = 5/4) analyzed blindly. Mann-Whitney test.

(B) Representative immunofluorescence images of wounds from Mr1+/+ and Mr1−/− animals (DAPI in blue, Ki67 in red). The white dashed line separates the epidermal tongue and the underlying dermis (left). Proliferation in the epidermis is quantified by the Ki67/DAPI ratio and normalized to the average expression in Mr1−/− animals for each experiment (right). Data are from two independent experiments (n = 5/6) analyzed blindly. Unpaired t test. Please also see Figure S6B.

(C) Dot plot showing RNA expression of repair molecules by non-cycling MAIT17 cells from integrated single-cell datasets as in Figure 1B.

(D) Feature plot of Areg expression projected on the UMAP of the integrated datasets.

(E) Ex vivo Areg staining on skin MAIT cells (blue: control skin; red: wound skin; gray: full staining except the biotinylated anti-Areg antibody). Pooled data from two independent experiments (n = 6). Wilcoxon test.

(F) Areg expression by thymic enriched MAIT cells following 36 h of in vitro activation by 5-OP-RU or IL-18. One experiment (n = 8) representative of 2. Dunn’s multiple comparison test.

(G) Wound surfaces at days 4 and 6 after excision on Zbtb16-cre−Aregfl/fl (black) and Zbtb16-cre+Aregfl/fl (gray). Pooled data from two independent experiments with one blind (full symbols) (ncre− = 8; ncre+ = 10). Mann-Whitney tests.

(H) Wound surfaces after excision (D5 and D7) of Cd3e−/− animals transferred with thymic MAIT cells expanded from Zbtb16-cre−Aregfl/fl (black) and Zbtb16-cre+Aregfl/fl (gray) littermate mice. Pooled data from two independent experiments with one blindly analyzed (full symbols) (ncre− = 9; ncre+ = 8). Mann-Whitney tests.