FIG. 5.

Fn-binding activity of GSTD1-4 and GSTD4 of CMRSA-1 compared to GSTD1-3 and GSTD3 from FnBPA of S. aureus 8325-4 (A) and recognition of GSTD1-4 by MAb 9C3 (B) or polyclonal antibody (C) specific for amino acids 20 to 33 of the D3 motif of S. aureus 8325-4. Wells of microtiter plates coated with 0.1 μg of fusion protein were incubated with various concentrations of biotinylated Fn (A), MAb 9C3 (B), or polyclonal antibody (C), which was obtained by immunizing with a synthetic peptide spanning amino acids 20 to 33 of the D3 motif. Each point represents the average (± standard deviation) of triplicate wells.