Table 1. Cost comparison between original4 vs. optimized sci-RNA-seq3 protocols.
We focus on the most expensive reagents here (all enzymes) as other reagent costs are comparatively negligible.
| Enzyme | Cost/μl | Original amount/plate | Original cost/plate | Optimized amount/plate | Optimized cost/plate |
|---|---|---|---|---|---|
| Superscript IV | $7.11 | 205 μl | $1458 | 55 μl | $391 |
| RNaseOUT | $1.56 | 205 μl | $320 | not used | $0 |
| SuperaseIN | $1.00 | 250 μl | $250 | not used | $0 |
| Quick Ligase | $2.59 | 215 μl | $557 | not used | $0 |
| T4 DNA Ligase | $1.04 | not used | $0 | 65 μl | $68 |
| USER | $1.21 | 110 μl | $133 | not used | $0 |
| Second Strand Synthesis | $2.95 | 73.3 μl | $216 | 35 μl | $103 |
| AmpureXP | $0.014 | 3840 μl | $53 | 230 μl | $3 |
| Tn5 | $24.50 | 0.92 μl | $22 | 4.6 μl | $112 |
| NEBNext 2X PCR mix | $0.058 | 1920 μl | $111 | 2200 μl | $127 |
| Total enzyme cost per plate (96×96×96) | $3,120 | $804 | |||
| Total enzyme cost per 4 plates (384×384×384) | $12,480 | $3,207 |