Figure 7. HFD feeding is sufficient to induce NASH development in Trem2ΔMye mice.
(A) Representative H&E, Sirius Red/Fast Green, and α-SMA staining of liver sections from Trem2F/F and Trem2ΔMye mice fed with HFD for 18 weeks, n = 6 mice per group. Sirius Red/Fast Green staining was detected under polarized light.
(B) Serum ALT, AST, hepatic hydroxyproline, and TNF amounts in liver tissue from mice as in (A) were measured. Relative mRNA expression of fibrosis-related genes, inflammatory genes, and Trem2 was analyzed by RT-qPCR using liver tissue, n = 6 mice per group.
(C) Representative Oil Red O staining of liver sections from mice as in (A). Scale bar, 100 μm. Body weight, liver weight, serum TG, liver TG, and total cholesterol from these mice were also analyzed. Relative mRNA expression of lipid-associated genes in liver tissue was measured by RT-qPCR. n = 6 mice per group.
(D) Apoptotic cells in liver tissue from mice as in (A) were stained with TUNEL and quantified. n = 6 mice per group.
(E) IPA on phagocytosis related functions in primary liver macrophages isolated from WT C57BL/6 mice that were fed with HFD (n=3) or ND (n=3) for 18 weeks.
(F) Heatmap of upregulated phagocytosis related genes (GO:0006909) in primary liver macrophages isolated from 18 weeks HFD (n=3) compared to ND (n=3) fed WT mice.
Scale bar, 100 μm. Data are shown as mean ± s.e.m.. *p<0.05; **p<0.01; ***p<0.001; NS, not significant. See also Figure S7.