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. Author manuscript; available in PMC: 2023 Jul 9.
Published in final edited form as: Cancer Discov. 2023 Jan 9;13(1):216–243. doi: 10.1158/2159-8290.CD-22-0561

Figure 5. MCD mutations trigger an aged/autoimmune-like program in GCBs.

Figure 5.

A, Differentially expressed genes in Myd88L252P/WT LZ GCB.

B, Pathway analysis for genes in (A).

C-D, FC analysis of (C) CD11c+ or (D) T-BET+ GCB.

E, GSEA of genes with a T-BET binding motif in their promoter (HOCOMOCO v11; >90% match within −5kb:TSS:+2kb), against Myd88L252P/WT LZ GCB.

F, GSEA of T-BET-KO MBC (GSE81189), against Myd88L252P/WT LZ GCB.

G, GSEA of canonical murine AiBC signatures (GSE175365), against Myd88L252P/WT LZ GCB.

H, GSEA of Tbl1xr1 mutant GCB (GSE139059), against Myd88L252P/WT LZ GCB.

I, RNA-Seq-based expression of genes of interest (G.O.I.) in Tbl1xr1 mutant (D370Y/WT) or WT GCB (GSE139059).

J, RT-qPCR validation of selected genes from (I), on independent animals.

K-L, FC analysis of (K) CD11c+ or (L) T-BET+ GCB in Tbl1xr1MUT mice.

M, GSEA of T-BET-KO MBC (GSE81189), against Tbl1xr1MUT GCB.

N, GSEA of canonical murine AiBC signatures, against Tbl1xr1MUT GCB.

Values represent mean ± SEM. P-values calculated using unpaired (D,J-L) or paired (C) two-tailed Student’s t-test.