Figure 1.

(a) Insulin secretion kinetics is not affected by Nkx6.1 ablation—insulin secretion was assayed in triplicates for control INS-1E^scrl/scrl cells (black) or lines (a) (yellow green) and (b) (green) of INS-1E^{Nkx6.1–/–} cells, when stimulated with 20 mM glucose adjustment after 1-h preincubation in the KRH medium, containing 3 mM glucose. Data were fit by linear regressions yielding GSIS rates of 101% ± 20% and 99% ± 30% for lines (a) and (b) relatively to INS-1E^scrl/scrl cells. (b) Nkx6.1 protein expression.