Table 1.
Primers employed for tested rat (r) genes—in forward (F) and reverse (R) direction.
| Gene | Forward primer | Gene | Reverse primer |
|---|---|---|---|
| r-Abcc8 197 F | ATCTACTGGACCCTGGCCTT | r-Abcc8 197 R | GGCTTTACTTCCCTTGGTGTC |
| r-Cacna1d 244 F | CTTTGGTACGGACGGCTCTC | r-Cacna1d 244 R | GCAGGGTATTTCCCCACCAG |
| r-Ero1lb 172 F | CGCCATCAACAGCACCCTAA | r-Ero1lb 172 R | TGAGGAGCCCTTGTAGCCAG |
| r-Glut2 245 F | GGCATGTTTTTCTGTGCCGT | r-Glut2 245 R | TACTGGAAGCAGAGGGCGAT |
| r-Ins1-g 100 F | CCCAAGTCCCGTCGTGAAGT | r-Ins1-g 100 R | CAACCTCCAGTGCCAAGGTC |
| r-Ins2-g 160 F | GTGACCAGCTACAGTCGGAA | r-Ins2-g 160 R | TTCCACCAAGTGAGAACCACAA |
| r-Kcnj11 206 F | ATCAGTCCAGAGGTTGGTGC | r-Kcnj11 206 R | GTACCTGGGCTCTGTAGGGT |
| r-Ldha 183 F | GTGCACTAAGCGGTCCCAAA | r-Ldha 183 R | GGCAAGCTCATCAGCCAAGT |
| r-MafA 105 F | ATCCGACTGAAACAGAAGCGG | r-MafA 105 R | GCACTTCTCGCTCTCCAGAAT |
| r-Mfn2 114 F | ACCTGAATCGGCACAGAGGA | r-Mfn2 114 R | GCAGGGACATCTCGTTTCTAGC |
| r-Nr4a1 213 F | CCAGATGCCTCCCCTACCAA | r-Nr4a1 213 R | GAAGCCCGGGATCTTTTCCG |
| r-Nr4a3 157 F | GATGAACGCCCTTGTCCGAG | r-Nr4a3 157 R | GCTTCTGGACACGTCGATGG |
| r-Nrf1 263 F | CATCCAGACGACGCAAGCAT | r-Nrf1 263 R | ATGCATGAACTCCATCTGGGC |
| r-Pcx 158 F | ATCTTGCACTTGTATGAGCGGG | r-Pcx 158 R | GTGCCTGCATTCTCATAGCCA |
| r-Pdx 152 F | ATCCACCTCCCGGACCTTTC | r-Pdx 152 R | CTCCGGTTCTGCTGCGTATG |
| r-Pgc1a 244 F | GACTGGCGTCATTCAGGAGC | r-Pgc1a 244 R | ATGTTCGCGGGCTCATTGTT |
| r-Polg 124 F | ATGCGAATGGTCCAGCGATTT | r-Polg 124 R | AACAGTTCCCGAGGCTCCTT |
| r-Slc30a8 99 F | CAAGCGGCTGACATTTGGGT | r-Slc30a8 99 R | GCAAGGTACACCAGCACACC |
| r-Ssbp1 243 F | GTGGCGATCAGGGGACAATG | r-Ssbp1 243 R | CAAGAAACGCTGCGTACCAC |
| r-Tfam F | GCATGATAACAAGCCCCTGGA | r-Tfam R | CCAGTGTTCTTAGCACGCCC |
| Reference gene | Forward primer | Reference gene | Reverse primer |
|---|---|---|---|
| r-Gusb F, glucuronidase, beta | AACAATCGGTTGCAGGGCTT | r-Gusb R | TCCCATTCACCCACACAACTG |
For a reference gene, rat glucuronidase beta (r-Gusb) was used.