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. 2023 Jan 13;14(1):26. doi: 10.1038/s41419-022-05534-w

Fig. 2. The upregulation of SF3B4 promotes migration and invasion of ccRCC cells.

Fig. 2

A, B RT-qPCR and Western blotting detected the SF3B4 expression in different cell lines of ccRCC. C Quantitative analysis of B. D, E Caki-1 cells were transfected with pLKO, shSF3B4-1#, or shSF3B4-2#, and then western blot analysis examined the SF3B4 and MMP1 expression. F, G 769-P cells were transfected with pWPI or pWPI-SF3B4 (oeSF3B4) and then SF3B4 and MMP1 were examined by western blot. H Caki-1 cells were treated as in D and transwell assay and detected the migration and invasion of cells. Scale bar = 50 μm. I Quantitative analysis of H. J 769-P cells was treated as in F and then the migration and invasion of cells were detected by transwell assay. K Quantitative analysis of J. L Caki-1 cells were treated as in D and a 3D Matrigel drop invasion assay detected the invasion of cells. Scale bar = 100 μm. M Quantitative analysis of L. N 769-P cells were treated as in F and a 3D Matrigel drop invasion assay detected the invasion of cells. O Quantitative analysis of N. All data are expressed as the mean ± SEM of three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001 vs. their corresponding controls.