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. 2023 Jan 13;14(1):26. doi: 10.1038/s41419-022-05534-w

Fig. 3. Twist1 mediates SF3B4-induced EMT in ccRCC.

Fig. 3

A Gene expression profiles in pLKO or shSF3B4-transfected Caki-1 cells. Heat map of hierarchical clustering indicates the differentially expressed genes (red: upregulation; blue: downregulation). B, C Caki-1 (B) and 769-P (C) cells were transfected with shSF3B4 or oeSF3B4, and then RT-qPCR detected the expression of the indicated genes. D The expressions of EMT-related genes, E-cadherin, vimentin, Twist1, and ZO-1 were detected by western blot in shSF3B4-transfected Caki-1 or oeSF3B4-transfected 769-P cells. E Quantitative analysis of D. F Caki cells were transfected with shSF3B4 or oeTeist1 or control vector, alone or together, and the expression of E-cadherin and vimentin were detected by double immunofluorescence staining. Scale bar = 20 μm. All data are expressed as the mean ± SEM of three independent experiments. *P < 0.05, **P < 0.01 vs. their corresponding controls.