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. 2023 Jan 13;14(1):26. doi: 10.1038/s41419-022-05534-w

Fig. 8. SF3B4-KLF16-Twist1 axis promotes the progression of ccRCC in vivo.

Fig. 8

A Caki-1 cells, engineered to stably knock down KLF16 or stably overexpress SF3B4, separately or together, were implanted into nude mice to establish xenograft tumors. The tumor sizes in each group were presented after 28 days. B Tumor volumes were measured by direct measurement with calipers. C Xenograft tumor wet weight was determined after the resection of tumors. D The expression of SF3B4, KLF16, Twist1, E-cadherin, and vimentin was examined in xenograft tumors by western blotting. E Quantitative analysis of B. F Double immunofluorescence staining of vimentin and E-cadherin in xenograft tumors. Scale bar = 20 μm. All data are expressed as the mean ± SEM of three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001 vs. their corresponding controls.