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. 2023 Jan 13;9:7. doi: 10.1038/s41420-023-01303-6

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — A IHC was performed with anti-NCKAP1 from TMA. Bar, 100 μm. Quantitative results on the right: the graph represents the score of NCKAP1. Data are reported as the mean ± SEM. One-way ANOVA and Bonferroni’s post hoc test. ns p > 0.05 vs. Stage I. (Stage I: n = 5, II: n = 16, III: n = 6, IV:3, Liver: n = 4, LN: n = 22). B The mRNA expression of NCKAP1 was confirmed by qRT-PCR analysis in blood buffy coats from patients with colon cancer. The bar graph below the dot graph represents the mean. Data are reported as the mean ± SEM. Student’s t-test. ns: non-significant (n = 10).