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. 2023 Jan 13;14:205. doi: 10.1038/s41467-023-35811-x

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a U2OS cells were actinomycin-D treated over an indicated period of time and the relative abundance of specific mRNA was analyzed. RT-qPCR quantification showed 3'UTR rG4 bearing mRNAs are significantly more stable under starvation stress, data are presented as mean values + /− SD, n = 3, Multiple paired t-tests, p-values are provided when p > 0.05. b Comparative mRNA stability in the presence of rG4 stabilizing ligands (cPDS and BRACO-19) showed a similar trend in mRNA stability as observed under starvation conditions, data are presented as mean values + /− SD, n = 3, ordinary One-way ANOVA, Multiple comparisons, adjusted p-values are provided when p > 0.05. c rG4 folding is reversible upon stress removal (also Supplementary Fig. S9) and such reversibility can be largely paused by rG4 stabilizing ligand cPDS, along with nutrient stresses created by 2-DG (glycolysis inhibitor) and CCCP (oxidative phosphorylation inhibitor) treatment. d Quantification of BG4 fluorescence from 10 cells per conditions in c and Supplementary Fig. S9, the experiment was performed in biological triplicate, the data presented as mean values + /− SD. Ordinary One-way ANOVA, Multiple comparisons, p-values are provided when p > 0.05, and e proposed model to demonstrate the dynamic nature of stress-responsive rG4 folding and their contribution to mRNA stability.