TABLE 1.
ELISA reactivity of antisera raised against recombinant Opc protein preparationsa
| Formulation | Reciprocal geometric mean ELISA titer (10−3) measured against:
|
|||||
|---|---|---|---|---|---|---|
| Homologous recombinant Opc protein | OMs from strain:
|
|||||
| MC58 Opc++ | MC139 Opc++ | MC139 Opc+ | H44/76 Opc+ | MC114 Opc− | ||
| Opc+Al(OH)3 | 3,990 | 76 | 944 | <1 | <1 | <1 |
| Opc-liposomes | 344 | 10 | 27 | <1 | <1 | <1 |
| Opc+MPLA-liposomes | 1,837 | 93 | 291 | <1 | 3 | 1 |
| Opc-liposomes+MPLA-liposomes | 778 | 78 | 64 | <1 | 4 | 1 |
| Opc-Zwit | 650 | 10 | 78 | <1 | <1 | <1 |
| Opc+MPLA-Zwit | 3,026 | 49 | 287 | <1 | 2 | <1 |
a
Mice were immunized with recombinant Opc protein in the preparations described in Materials and Methods. Pre- and postimmunization sera were tested in ELISA against homologous protein and against OMs from the homologous and heterologous strains. Strains MC58 and H44/76 expressed the homologous Opc protein, strain MC139 expressed a heterologous protein; the levels of expression were high (Opc++), low (Opc+), and none (Opc−). Preimmune sera and antisera raised against control liposomes with and without MPLA showed no reactivity against antigens in the ELISA (<1).