TABLE 2.
Antibody subclass-specific response to homologous Opc protein following immunization of mice with recombinant Opc protein preparationsa
| Formulation | Mean conc of antibody isotype (μg ml−1)
|
||||
|---|---|---|---|---|---|
| IgG1 | IgG2a | IgG2b | IgG3 | IgM | |
| Opc+Al(OH)3 | 546 | <0.1 | <0.1 | <0.1 | 47 |
| Opc-liposomes | 33 | <0.1 | 4 | 1 | 6 |
| Opc+MPLA-liposomes | 49 | 32 | 9 | 1 | 7 |
| Opc-liposomes+MPLA-liposomes | 216 | 14 | 15 | 4 | 6 |
| Opc-Zwit | 114 | <0.1 | 2 | <0.1 | 3 |
| Opc+MPLA-Zwit | 82 | 32 | 12 | <0.1 | 7 |
| Liposomes (empty) | <0.1 | <0.1 | <0.1 | <0.1 | <0.1 |
| MPLA-liposomes | <0.1 | <0.1 | <0.1 | <0.1 | 5 |
| Normal mouse serum | 0.2 | <0.1 | <0.1 | <0.1 | 2 |
a
ELISA with enzyme conjugates of rat MAbs specific for murine Ig subclasses was used to determine concentrations of antibody subclasses in murine antisera. Data are represented as the mean antibody isotype concentration in serum for groups of five individual mice. A concentration of <0.1 μg (ml of serum)−1 was below the minimum detection limit.