PPARγ acetylation is dynamically regulated in metabolic changes. a) Western blot (WB) analysis and quantification of PPARγ acetylation levels in BAT (n = 4, 4) and eWAT (n = 5, 5) in young (8 weeks) and aging (40 weeks) mice. b) WB analysis and quantification of PPARγ acetylation levels in BAT of chow‐fed and DIO mice (n = 4,3). c,d) WB analyses and quantifications of PPARγ acetylation levels in the eWAT of chow‐fed, DIO, and aging (1‐year‐old) mice sacrificed at 6 h intervals throughout the day (n = 3). e) Schematic design for shiftwork mouse model. f) WB analysis and quantification of PPARγ acetylation levels in BAT (n = 4, 4) and eWAT (n = 4, 4) of shiftwork mice and control mice. Data are presented as mean ± SEM, two‐tailed Student's t‐test. *p < 0.05, **p < 0.01, and ***p < 0.001.